Hotspot

psbE-petL

LSC · 64717-65846 · 1130 bp

Back to Homalium

Pi 0.025

MarkerSeek score 73.80

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1130
variable_sites: 31
pi: 0.025
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 73.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	AGAACGTTCTCCTGTGCTTC	AGTCGTATCTTGCTCAGACT	50.0	45.0	57.8	55.7	923-1246 bp	1.0	66.6
psbE-petL_p2	GTTCTCCTGTGCTTCCAGAC	AGTCGTATCTTGCTCAGACT	55.0	45.0	58.2	55.7	918-1241 bp	1.0	66.3
psbE-petL_p3	TCTCCTGTGCTTCCAGACAT	AGTCGTATCTTGCTCAGACT	50.0	45.0	58.4	55.7	916-1239 bp	1.0	65.1
psbE-petL_p4	AGAACGTTCTCCTGTGCTTC	AAGTCGTATCTTGCTCAGACT	50.0	42.9	57.8	56.5	924-1247 bp	1.0	64.9
psbE-petL_p5	GTTCTCCTGTGCTTCCAGAC	AAGTCGTATCTTGCTCAGACT	55.0	42.9	58.2	56.5	919-1242 bp	1.0	64.6