Hotspot

atpB-rbcL

LSC · 54426-55217 · 792 bp

Back to Homalium

Pi 0.00608

MarkerSeek score 65.40

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 792
variable_sites: 7
pi: 0.00608
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 65.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	TTTTTCAAGCGCGGAAACTC	GCCTTGAATCCAACACTTGC	45.0	50.0	57.9	57.9	884-908 bp	1.0	81.3
atpB-rbcL_p2	TTTTTCAAGCGCGGAAACTC	TTTGTGGTGACATAAGCCCC	45.0	50.0	57.9	58.1	853-877 bp	1.0	81.3
atpB-rbcL_p3	TTTTTCAAGCGCGGAAACTC	CCAGCCTTGAATCCAACACT	45.0	50.0	57.9	58.1	887-911 bp	1.0	81.2
atpB-rbcL_p4	TTTTTCAAGCGCGGAAACTC	TAAGCCCCTCCCTACAACTC	45.0	55.0	57.9	58.1	841-865 bp	1.0	81.0
atpB-rbcL_p5	GAGCGATATGTCCCAGGTTC	GCCTTGAATCCAACACTTGC	55.0	50.0	57.8	57.9	906-930 bp	1.0	80.9