Hotspot

ndhC-trnV

LSC · 50184-51336 · 1153 bp

Back to Homalium

Pi 0.000599

MarkerSeek score 62.40

Primer available yes

Label ndhC-trnV

Feature Metadata

label_name: ndhC-trnV
feature_type: igs
region: LSC
length_bp: 1153
variable_sites: 1
pi: 0.000599
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 62.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV_p1	GGGGATAAAAGGCCGGAAAT	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.9	57.8	1284-1479 bp	1.0	80.1
ndhC-trnV_p2	AATTGGGGATAAAAGGCCGG	AGAAGGTCTACGGTTCGAGT	50.0	50.0	58.2	57.8	1288-1483 bp	1.0	79.1
ndhC-trnV_p3	TGGCCCTTTTCTAATTGGGG	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.8	57.8	1300-1495 bp	1.0	78.8
ndhC-trnV_p4	GGGGATAAAAGGCCGGAAAT	GAAGGTCTACGGTTCGAGTC	50.0	55.0	57.9	57.5	1283-1478 bp	1.0	77.4
ndhC-trnV_p5	GGCCCTTTTCTAATTGGGGA	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.5	57.8	1299-1494 bp	1.0	76.4