Hotspot

psaA-ycf3

LSC · 41702-42352 · 651 bp

Back to Homalium

Pi 0.00205

MarkerSeek score 63.60

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 651
variable_sites: 2
pi: 0.00205
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GCCCATTCCTCGAAAGAAGT	AGCGCAGAATTGGTTGAAGA	50.0	45.0	57.9	58.1	771-905 bp	1.0	80.3
psaA-ycf3_p2	GCCCATTCCTCGAAAGAAGT	AAGATCACAAGGCGTTTCGA	50.0	45.0	57.9	57.8	755-889 bp	1.0	79.9
psaA-ycf3_p3	GCCCATTCCTCGAAAGAAGT	AGATCACAAGGCGTTTCGAA	50.0	45.0	57.9	57.8	754-888 bp	1.0	79.9
psaA-ycf3_p4	GCCCATTCCTCGAAAGAAGT	GCTATAGCGCTTACTCCTGG	50.0	55.0	57.9	57.6	803-937 bp	1.0	78.2
psaA-ycf3_p5	GCCCATTCCTCGAAAGAAGT	TGAAGCGCAGAATTGGTTGA	50.0	45.0	57.9	58.4	774-908 bp	1.0	78.0