Hotspot

psbZ-trnG

LSC · 35814-36305 · 492 bp

Back to Homalium

Pi 0.0276

MarkerSeek score 62.90

Primer available yes

Label psbZ-trnG

Feature Metadata

label_name: psbZ-trnG
feature_type: igs
region: LSC
length_bp: 492
variable_sites: 16
pi: 0.0276
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 62.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG_p1	TAGGAGTTGATACCGTCGCT	TTCTTCTCTTCATCACGCGG	50.0	50.0	58.0	58.0	1274-1350 bp	1.0	85.0
psbZ-trnG_p2	TAGGAGTTGATACCGTCGCT	TGGAAAGTTACAAGCCCCAC	50.0	50.0	58.0	58.0	1596-1672 bp	1.0	84.9
psbZ-trnG_p3	CAAGACCGGAGCTATCAACC	TTCTTCTCTTCATCACGCGG	55.0	50.0	58.1	58.0	1367-1443 bp	1.0	84.8
psbZ-trnG_p4	TAGGAGTTGATACCGTCGCT	GAAAGTTACAAGCCCCACCA	50.0	50.0	58.0	58.0	1594-1670 bp	0.666667	70.2
psbZ-trnG_p5	CGAATAAAGTGGGAAGGGGG	TTCTTCTCTTCATCACGCGG	55.0	50.0	57.9	58.0	1142-1166 bp	0.666667	69.3