Hotspot

trnK-trnH

LSC · 4415-5688 · 1274 bp

Back to Homalium

Pi 0.00851

MarkerSeek score 66.40

Primer available yes

Label trnK-trnH

Feature Metadata

label_name: trnK-trnH
feature_type: igs
region: LSC
length_bp: 1274
variable_sites: 16
pi: 0.00851
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 66.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-trnH_p1	AAAGCTGTTAGCCGCACTTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	57.8	58.1	1352-2146 bp	1.0	80.1
trnK-trnH_p2	AAGCTGTTAGCCGCACTTAA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	57.8	58.1	1351-2145 bp	1.0	80.1
trnK-trnH_p3	AGCTGTTAGCCGCACTTAAA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	57.8	58.1	1350-2144 bp	1.0	80.1
trnK-trnH_p4	TAAAAGCTGTTAGCCGCACT	GAGGTTCGAATCCTTCCGTC	45.0	55.0	57.8	58.1	1354-2148 bp	1.0	80.1
trnK-trnH_p5	GTTAGCCGCACTTAAAAGCC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.7	58.1	1346-2140 bp	1.0	79.4