Hotspot

atpI

LSC · 15991-16734 · 744 bp

Back to Holoptelea

Pi 0

MarkerSeek score 40.00

Primer available yes

Label atpI

Feature Metadata

label_name: atpI
feature_type: gene
region: LSC
length_bp: 744
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpI_p1	TCTTCCCTAAACCTAAGCGGA	AGGCAAGTCGAAAAAGAGATGA	47.6	40.9	58.2	58.0	898 bp	1.0	54.5
atpI_p2	CTTCCCTAAACCTAAGCGGA	AGGCAAGTCGAAAAAGAGATGA	50.0	40.9	56.6	58.0	897 bp	1.0	53.0
atpI_p3	TCTTCCCTAAACCTAAGCGG	AGGCAAGTCGAAAAAGAGATGA	50.0	40.9	56.6	58.0	898 bp	1.0	53.0
atpI_p4	TCTTCCCTAAACCTAAGCGGA	GGCAAGTCGAAAAAGAGATGA	47.6	42.9	58.2	56.6	897 bp	1.0	51.0
atpI_p5	TCTTCCCTAAACCTAAGCGGA	AGGCAAGTCGAAAAAGAGATG	47.6	42.9	58.2	56.5	898 bp	1.0	50.9