Hotspot

rps16-trnQ-UUG

LSC · 6558-7586 · 1029 bp

Back to Holoptelea

Pi 0
MarkerSeek score 40.00
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1029
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 1102 bp 1.0 78.3
rps16-trnQ-UUG_p2 CCACAACAGATCGTGTCCTT GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.8 58.1 1123 bp 1.0 78.0
rps16-trnQ-UUG_p3 AAGTCGCACGTTGCTTTCTA TAAGGCATCGGGTTTTGGTC 45.0 50.0 58.1 58.2 1132 bp 1.0 77.5
rps16-trnQ-UUG_p4 CCACAACAGATCGTGTCCTT TAAGGCATCGGGTTTTGGTC 50.0 50.0 57.8 58.2 1153 bp 1.0 77.3
rps16-trnQ-UUG_p5 CTCCACAACAGATCGTGTCC GAGGTTCGAATCCTTCCGTC 55.0 55.0 58.3 58.1 1125 bp 1.0 77.1