Hotspot

rpoC2

LSC · 24841-29262 · 4422 bp

Pi 0.000226

MarkerSeek score 53.50

Primer available yes

Label rpoC2

Feature Metadata

label_name: rpoC2
feature_type: gene
region: LSC
length_bp: 4422
variable_sites: 1
pi: 0.000226
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2_p1	CATTGCCGCTCGAAATCAAG	CCTACCGCTTCTCCCAATTC	50.0	55.0	58.2	58.0	198 bp	1.0	78.6
rpoC2_p2	TTGCCGCTCGAAATCAAGAT	CCTACCGCTTCTCCCAATTC	45.0	55.0	58.3	58.0	196 bp	1.0	78.0
rpoC2_p3	ATTGCCGCTCGAAATCAAGA	CCTACCGCTTCTCCCAATTC	45.0	55.0	58.3	58.0	197 bp	1.0	77.9
rpoC2_p4	CATTGCCGCTCGAAATCAAG	ATACCTACCGCTTCTCCCAA	50.0	50.0	58.2	57.8	201 bp	1.0	77.6
rpoC2_p5	CATTGCCGCTCGAAATCAAG	TACCTACCGCTTCTCCCAAT	50.0	50.0	58.2	57.8	200 bp	1.0	77.6