Hotspot

clpP

LSC · 67341-67991 · 651 bp

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Pi 0.00154
MarkerSeek score 59.60
Primer available yes
Label clpP

Feature Metadata

label_name
clpP
feature_type
gene
region
LSC
length_bp
651
variable_sites
1
pi
0.00154
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
clpP_p1 TCGAAGAAAACCCGAATTTGG ACTGCGGATTCTTTCTTTCTCT 42.9 40.9 57.1 57.7 790 bp 1.0 46.6
clpP_p2 TCGAAGAAAACCCGAATTTGG GCGGATTCTTTCTTTCTCTTCC 42.9 45.5 57.1 57.4 787 bp 1.0 44.0
clpP_p3 TCGAAGAAAACCCGAATTTGG ACTGCGGATTCTTTCTTTCTC 42.9 42.9 57.1 56.3 790 bp 1.0 43.0
clpP_p4 CGAAGAAAACCCGAATTTGG ACTGCGGATTCTTTCTTTCTCT 45.0 40.9 55.6 57.7 789 bp 1.0 42.4
clpP_p5 ATCGAAGAAAACCCGAATTTGG ACTGCGGATTCTTTCTTTCTCT 40.9 40.9 57.6 57.7 791 bp 1.0 42.4