Hotspot

rpl32-trnL-UAG

SSC · 105030-105647 · 618 bp

Back to Holcus

Pi 0.00162
MarkerSeek score 59.70
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
618
variable_sites
1
pi
0.00162
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 AAGGGTTTTTCTGGGCAACA TTCGAACCGAGATGCTTGAG 45.0 50.0 58.1 58.0 717 bp 1.0 79.1
rpl32-trnL-UAG_p2 AGGGTTTTTCTGGGCAACAA TTCGAACCGAGATGCTTGAG 45.0 50.0 58.1 58.0 716 bp 1.0 79.1
rpl32-trnL-UAG_p3 AAGGGTTTTTCTGGGCAACA GGATTCGAACCGAGATGCTT 45.0 50.0 58.1 58.1 720 bp 1.0 78.6
rpl32-trnL-UAG_p4 AGGGTTTTTCTGGGCAACAA GGATTCGAACCGAGATGCTT 45.0 50.0 58.1 58.1 719 bp 1.0 78.6
rpl32-trnL-UAG_p5 AGGGTTTTTCTGGGCAACAA TCCTAAGAGCAGCGTGTCTA 45.0 50.0 58.1 57.9 689 bp 1.0 78.1