Hotspot

rbcL-psaI

LSC · 55732-57139 · 1408 bp

Back to Holcus

Pi 0.00213
MarkerSeek score 60.00
Primer available yes
Label rbcL-psaI

Feature Metadata

label_name
rbcL-psaI
feature_type
igs
region
LSC
length_bp
1408
variable_sites
3
pi
0.00213
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rbcL-psaI_p1 AGTTCGAGCCGGTAGATACT GTCATTGCAATTGCCGGAAA 50.0 45.0 57.7 57.9 1511 bp 1.0 76.8
rbcL-psaI_p2 GGCGATCAAATTCGAGTTCG GTCATTGCAATTGCCGGAAA 50.0 45.0 57.6 57.9 1525 bp 1.0 76.7
rbcL-psaI_p3 TGGAAGGCGATCAAATTCGA GTCATTGCAATTGCCGGAAA 45.0 45.0 57.6 57.9 1530 bp 1.0 76.4
rbcL-psaI_p4 AGCTTGCAAATGGAGTCCTG GTCATTGCAATTGCCGGAAA 50.0 45.0 58.5 57.9 1573 bp 1.0 75.9
rbcL-psaI_p5 CAGCTTGCAAATGGAGTCCT GTCATTGCAATTGCCGGAAA 50.0 45.0 58.5 57.9 1574 bp 1.0 75.9