Hotspot

trnC-GCA-rpoB

LSC · 18162-19339 · 1178 bp

Back to Holcus

Pi 0.0017
MarkerSeek score 59.80
Primer available yes
Label trnC-GCA-rpoB

Feature Metadata

label_name
trnC-GCA-rpoB
feature_type
igs
region
LSC
length_bp
1178
variable_sites
2
pi
0.0017
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnC-GCA-rpoB_p1 AAAGGATTTGCAGTCCCCTG ATTCCCTCGTTTCCATTCCG 50.0 50.0 58.1 58.0 1246-1247 bp 1.0 79.3
trnC-GCA-rpoB_p2 AAAGGATTTGCAGTCCCCTG TGTGGACATTCCCTCGTTTC 50.0 50.0 58.1 57.8 1253-1254 bp 1.0 78.2
trnC-GCA-rpoB_p3 AAAGGATTTGCAGTCCCCTG TGTCAAGTTCTTCTGCCAGG 50.0 50.0 58.1 57.7 1329-1330 bp 1.0 77.5
trnC-GCA-rpoB_p4 AAAGGATTTGCAGTCCCCTG CCGGGTATTGTGGACATTCC 50.0 55.0 58.1 58.3 1261-1262 bp 1.0 77.0
trnC-GCA-rpoB_p5 AAAGGATTTGCAGTCCCCTG CGGGTATTGTGGACATTCCC 50.0 55.0 58.1 58.3 1260-1261 bp 1.0 77.0