Hotspot

trnS-UGA-psbZ

LSC · 11324-11675 · 352 bp

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Pi 0
MarkerSeek score 34.80
Primer available yes
Label trnS-UGA-psbZ

Feature Metadata

label_name
trnS-UGA-psbZ
feature_type
igs
region
LSC
length_bp
352
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
34.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-UGA-psbZ_p1 CAACCACTCAGCCATCTCTC GGCTGAGGAAGTCGCAATTA 55.0 50.0 58.0 58.0 427 bp 1.0 79.6
trnS-UGA-psbZ_p2 GAGCTATCAACCACTCAGCC GGCTGAGGAAGTCGCAATTA 55.0 50.0 58.1 58.0 434 bp 1.0 79.4
trnS-UGA-psbZ_p3 GGAGCTATCAACCACTCAGC GGCTGAGGAAGTCGCAATTA 55.0 50.0 58.1 58.0 435 bp 1.0 79.4
trnS-UGA-psbZ_p4 CAAGACCGGAGCTATCAACC GGCTGAGGAAGTCGCAATTA 55.0 50.0 58.1 58.0 442 bp 1.0 79.3
trnS-UGA-psbZ_p5 CAACCACTCAGCCATCTCTC CCAACCATCAGGAGAAGCAA 55.0 50.0 58.0 57.8 472 bp 1.0 78.2