Hotspot

rpl16

LSC · 81335-83045 · 1711 bp

Back to Holcoglossum

Pi 0.0163

MarkerSeek score 59.60

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1711
variable_sites: 69
pi: 0.0163
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	ATTGGGCACTGCTTCTTTGA	GCAAATTGCAGGACGTATCG	45.0	50.0	58.0	57.9	2138-2328 bp	1.0	86.3
rpl16_p2	TGGGCACTGCTTCTTTGATT	GCAAATTGCAGGACGTATCG	45.0	50.0	58.0	57.9	2136-2326 bp	1.0	86.3
rpl16_p3	TTGGGCACTGCTTCTTTGAT	GCAAATTGCAGGACGTATCG	45.0	50.0	58.0	57.9	2137-2327 bp	1.0	86.3
rpl16_p4	TCGCCAAGATGAGCATATCG	GCAAATTGCAGGACGTATCG	50.0	50.0	57.9	57.9	2101-2291 bp	1.0	85.8
rpl16_p5	TATCATGCCGTTGTCACGTT	GCAAATTGCAGGACGTATCG	45.0	50.0	57.9	57.9	2219-2409 bp	1.0	85.5