Hotspot

ndhD

SSC · 112698-113861 · 1164 bp

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Pi 0.0215

MarkerSeek score 60.60

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1164
variable_sites: 78
pi: 0.0215
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 60.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	AGGCATTCTTTCTGGAGCAG	AGATGTGAATCCGCTTGTCC	50.0	50.0	57.9	58.0	1660-1979 bp	1.0	87.6
ndhD_p2	GGCATTCTTTCTGGAGCAGT	AGATGTGAATCCGCTTGTCC	50.0	50.0	58.2	58.0	1659-1978 bp	1.0	87.3
ndhD_p3	TCTGGAGCAGTATGGGCTAA	AGATGTGAATCCGCTTGTCC	50.0	50.0	57.8	58.0	1650-1969 bp	1.0	87.2
ndhD_p4	AGGCATTCTTTCTGGAGCAG	GCACGGGCTTTTCTGGTATA	50.0	50.0	57.9	58.0	1478-1797 bp	0.846154	81.4
ndhD_p5	GGCATTCTTTCTGGAGCAGT	GCACGGGCTTTTCTGGTATA	50.0	50.0	58.2	58.0	1477-1796 bp	0.846154	81.0