Hotspot

psaC-ndhE

SSC · 114237-114963 · 727 bp

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Pi 0.0388

MarkerSeek score 65.30

Primer available yes

Label psaC-ndhE

Feature Metadata

label_name: psaC-ndhE
feature_type: igs
region: SSC
length_bp: 727
variable_sites: 70
pi: 0.0388
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.83
markerseek_score: 65.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhE_p1	GTCCATCGGTTTCCAGTCTC	GTCGAAGCATGGCTAGAACA	55.0	50.0	58.0	58.0	1462-1621 bp	0.923077	86.1
psaC-ndhE_p2	GTCCATCGGTTTCCAGTCTC	TCGAAGCATGGCTAGAACAC	55.0	50.0	58.0	58.0	1461-1620 bp	0.923077	86.1
psaC-ndhE_p3	GTCCATCGGTTTCCAGTCTC	CGAAGCATGGCTAGAACACT	55.0	50.0	58.0	58.0	1460-1619 bp	0.923077	86.1
psaC-ndhE_p4	GGACAAGCGGATTCACATCT	TCGAAGCATGGCTAGAACAC	50.0	50.0	58.0	58.0	976-1138 bp	0.923077	86.1
psaC-ndhE_p5	GTCCATCGGTTTCCAGTCTC	ACAAGTCGAAGCATGGCTAG	55.0	50.0	58.0	58.0	1474-1625 bp	0.769231	79.5