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clpP-psbB

LSC · 71457-72200 · 744 bp

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Pi 0.0518

MarkerSeek score 96.10

Primer available yes

Label clpP-psbB

Feature Metadata

label_name: clpP-psbB
feature_type: igs
region: LSC
length_bp: 744
variable_sites: 104
pi: 0.0518
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 96.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP-psbB_p1	CGCACTATACGTCAACCCAA	CAACCAGAAACCAGAGCTGT	50.0	50.0	58.0	58.0	804-1050 bp	1.0	91.8
clpP-psbB_p2	CTTTTGGAACACCAATGGGC	CAACCAGAAACCAGAGCTGT	50.0	50.0	57.8	58.0	750-996 bp	1.0	90.4
clpP-psbB_p3	ACCCAAACTGCATCTTCCTC	CAACCAGAAACCAGAGCTGT	50.0	50.0	57.8	58.0	790-1036 bp	1.0	90.1
clpP-psbB_p4	CGCACTATACGTCAACCCAA	CGATACCAAGGCAAACCCAT	50.0	50.0	58.0	58.2	726-972 bp	1.0	90.1
clpP-psbB_p5	TCCTCTCCAGGATTCCGAAA	CAACCAGAAACCAGAGCTGT	50.0	50.0	57.8	58.0	775-1021 bp	1.0	89.8