Hotspot

trnT-GGU-psbD

LSC · 33495-34530 · 1036 bp

Back to Holcoglossum

Pi 0.016

MarkerSeek score 61.60

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1036
variable_sites: 52
pi: 0.016
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GGCGTAAGTCATCGGTTCAA	TCTACCAAAGGCTATAGTCA	50.0	40.0	58.3	52.4	1042-1102 bp	1.0	47.9
trnT-GGU-psbD_p2	GTGGTAGAGTAACGCCATGG	TCTACCAAAGGCTATAGTCA	55.0	40.0	58.1	52.4	1065-1125 bp	1.0	47.8
trnT-GGU-psbD_p3	TCAGTGGTAGAGTAACGCCA	TCTACCAAAGGCTATAGTCA	50.0	40.0	58.1	52.4	1068-1128 bp	1.0	47.8
trnT-GGU-psbD_p4	GTGGTAGAGTAACGCCATGG	TCTACCAAAGGCTATAGTCAT	55.0	38.1	58.1	53.2	1065-1125 bp	1.0	47.8
trnT-GGU-psbD_p5	GGCCCTTTTAACTCAGTGGT	TCTACCAAAGGCTATAGTCA	50.0	40.0	57.7	52.4	1080-1140 bp	1.0	47.7