Hotspot

matK-trnK-UUU

LSC · 3264-4393 · 1130 bp

Back to Holcoglossum

Pi 0.0153

MarkerSeek score 67.30

Primer available yes

Label matK-trnK-UUU

Feature Metadata

label_name: matK-trnK-UUU
feature_type: igs
region: LSC
length_bp: 1130
variable_sites: 71
pi: 0.0153
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-trnK-UUU_p1	TCCTGAAGGAGTAGCGGATA	TCCGGGTTGCTAACTCAATG	50.0	50.0	57.0	57.9	1171-1306 bp	1.0	79.4
matK-trnK-UUU_p2	TCCTGAAGGAGTAGCGGATA	CGGGTTGCTAACTCAATGGT	50.0	50.0	57.0	58.2	1169-1304 bp	1.0	78.8
matK-trnK-UUU_p3	TCCTGAAGGAGTAGCGGATA	GATCCGGGTTGCTAACTCAA	50.0	50.0	57.0	57.6	1173-1308 bp	1.0	77.0
matK-trnK-UUU_p4	TCCTGAAGGAGTAGCGGATA	TTGATCCGGGTTGCTAACTC	50.0	50.0	57.0	57.6	1175-1310 bp	1.0	77.0
matK-trnK-UUU_p5	TCCTGAAGGAGTAGCGGATAT	TCCGGGTTGCTAACTCAATG	47.6	50.0	57.5	57.9	1171-1306 bp	1.0	75.5