Hotspot

ycf2

IRa · 148687-155577 · 6891 bp

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Pi 0

MarkerSeek score 33.80

Primer available yes

Label ycf2

Feature Metadata

label_name: ycf2
feature_type: gene
region: IRa
length_bp: 6891
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 33.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2_p1	CTACCACCCGGTCAATTCTG	GTACTTCGAGCTTGGAACGA	55.0	50.0	58.0	57.7	1129 bp	1.0	77.1
ycf2_p2	TCTACCACCCGGTCAATTCT	GTACTTCGAGCTTGGAACGA	50.0	50.0	58.1	57.7	1130 bp	1.0	76.8
ycf2_p3	GTTTCCACGACTCTACCACC	GTACTTCGAGCTTGGAACGA	55.0	50.0	57.9	57.7	1141 bp	1.0	76.6
ycf2_p4	CTACCACCCGGTCAATTCTG	TGGTACTTCGAGCTTGGAAC	55.0	50.0	58.0	57.5	1131 bp	1.0	76.2
ycf2_p5	AATCCCTCTTTCATGGCCAC	GTACTTCGAGCTTGGAACGA	50.0	50.0	57.9	57.7	1101 bp	1.0	76.1