Hotspot

ycf1

SSC · 127095-132686 · 5592 bp

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Pi 0.000238

MarkerSeek score 52.70

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5592
variable_sites: 2
pi: 0.000238
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	AAAATCTCCTTGGCCTGCTT	AAGATTCAACCGTGGCTCAT	45.0	45.0	57.7	57.5	198 bp	1.0	73.9
ycf1_p2	AAATCTCCTTGGCCTGCTTG	AAGATTCAACCGTGGCTCAT	50.0	45.0	58.4	57.5	197 bp	1.0	72.9
ycf1_p3	CAAAATCTCCTTGGCCTGCT	AAGATTCAACCGTGGCTCAT	50.0	45.0	58.4	57.5	199 bp	1.0	72.9
ycf1_p4	AAAATCTCCTTGGCCTGCTT	TCCCATGTCGAATCTGGAAC	45.0	50.0	57.7	57.3	259 bp	1.0	72.3
ycf1_p5	AAAATCTCCTTGGCCTGCTT	CCCATGTCGAATCTGGAACT	45.0	50.0	57.7	57.3	258 bp	1.0	72.2