Hotspot

rps3

LSC · 85097-85753 · 657 bp

Back to Holboellia

Pi 0.00101

MarkerSeek score 46.90

Primer available yes

Label rps3

Feature Metadata

label_name: rps3
feature_type: gene
region: LSC
length_bp: 657
variable_sites: 1
pi: 0.00101
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps3_p1	CCATTGCTGGATAGAACGGA	ACTGAAATGGGCTACTAGCG	50.0	50.0	57.4	57.4	724 bp	1.0	70.3
rps3_p2	TCCATTGCTGGATAGAACGG	ACTGAAATGGGCTACTAGCG	50.0	50.0	57.4	57.4	725 bp	1.0	70.3
rps3_p3	CCATTGCTGGATAGAACGGA	ATCGGGACAGACTGAAATGG	50.0	50.0	57.4	57.3	734 bp	1.0	69.6
rps3_p4	TCCATTGCTGGATAGAACGG	ATCGGGACAGACTGAAATGG	50.0	50.0	57.4	57.3	735 bp	1.0	69.6
rps3_p5	CCATTGCTGGATAGAACGGA	TGAAATGGGCTACTAGCGAG	50.0	50.0	57.4	57.1	722 bp	1.0	68.0