Hotspot

ccsA

SSC · 117481-118452 · 972 bp

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Pi 0

MarkerSeek score 31.10

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 972
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 31.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	CCCTCCTCTTTGTGCGTTTA	TCTCAAAAACTCACACACGC	50.0	45.0	57.8	56.9	1075 bp	1.0	69.7
ccsA_p2	CCCTCCTCTTTGTGCGTTTA	CTCAAAAACTCACACACGCT	50.0	45.0	57.8	56.9	1074 bp	1.0	69.7
ccsA_p3	CCCTCCTCTTTGTGCGTTTA	TCTCAAAAACTCACACACGCT	50.0	42.9	57.8	58.4	1075 bp	1.0	67.6
ccsA_p4	CCCTCCTCTTTGTGCGTTTA	TTCTCAAAAACTCACACACGC	50.0	42.9	57.8	57.6	1076 bp	1.0	67.4
ccsA_p5	CCCTCCTCTTTGTGCGTTTA	CTCAAAAACTCACACACGCTT	50.0	42.9	57.8	57.6	1074 bp	1.0	67.3