Hotspot

ccsA-ndhD

SSC · 118453-118669 · 217 bp

Back to Holboellia

Pi 0.0154

MarkerSeek score 49.80

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 217
variable_sites: 5
pi: 0.0154
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	GCAATTGTGGCTTCTATCGG	AGTTGACAAGGTGGAGGCTA	50.0	50.0	57.2	58.3	374 bp	1.0	72.8
ccsA-ndhD_p2	GCAATTGTGGCTTCTATCGG	GTTGACAAGGTGGAGGCTAT	50.0	50.0	57.2	57.2	373 bp	1.0	68.7
ccsA-ndhD_p3	CTGCAATTGTGGCTTCTATCG	AGTTGACAAGGTGGAGGCTA	47.6	50.0	57.7	58.3	376 bp	1.0	68.3
ccsA-ndhD_p4	GCAATTGTGGCTTCTATCGG	ATCAGTTGACAAGGTGGAGG	50.0	50.0	57.2	57.1	377 bp	1.0	68.0
ccsA-ndhD_p5	TGCAATTGTGGCTTCTATCG	AGTTGACAAGGTGGAGGCTA	45.0	50.0	56.5	58.3	375 bp	1.0	66.9