Hotspot

atpB-rbcL

LSC · 56111-56870 · 760 bp

Back to Holboellia

Pi 0.000877

MarkerSeek score 46.80

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 760
variable_sites: 1
pi: 0.000877
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	GGTGGAAAGGCTACATCCAG	CACCAGCTTTGAATCCAACAC	55.0	47.6	58.0	58.0	919-920 bp	1.0	71.6
atpB-rbcL_p2	CATCCAGTACCGGACCAATG	CACCAGCTTTGAATCCAACAC	55.0	47.6	58.0	58.0	906-907 bp	1.0	71.6
atpB-rbcL_p3	GGTGGAAAGGCTACATCCAG	ACCAGCTTTGAATCCAACACT	55.0	42.9	58.0	58.1	918-919 bp	1.0	71.3
atpB-rbcL_p4	CATCCAGTACCGGACCAATG	ACCAGCTTTGAATCCAACACT	55.0	42.9	58.0	58.1	905-906 bp	1.0	71.3
atpB-rbcL_p5	TTTGAGCAATACGACCCAGG	CACCAGCTTTGAATCCAACAC	50.0	47.6	57.9	58.0	885-886 bp	1.0	71.1