Hotspot

rps4-trnT-UGU

LSC · 48136-48514 · 379 bp

Back to Holboellia

Pi 0

MarkerSeek score 54.70

Primer available yes

Label rps4-trnT-UGU

Feature Metadata

label_name: rps4-trnT-UGU
feature_type: igs
region: LSC
length_bp: 379
variable_sites: 0
pi: 0
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnT-UGU_p1	TTCCGGGTCTAGGCCTTTTA	CTCAGAGGTTAGAGCATCGC	50.0	55.0	58.0	57.9	528-530 bp	1.0	78.9
rps4-trnT-UGU_p2	AGATCACTTCCGGGTCTAGG	CTCAGAGGTTAGAGCATCGC	55.0	55.0	57.9	57.9	535-537 bp	1.0	78.6
rps4-trnT-UGU_p3	TTCCGGGTCTAGGCCTTTTA	ATGGTCATCGGTTCGATTCC	50.0	50.0	58.0	57.8	496-498 bp	1.0	78.0
rps4-trnT-UGU_p4	AGATCACTTCCGGGTCTAGG	ATGGTCATCGGTTCGATTCC	55.0	50.0	57.9	57.8	503-505 bp	1.0	77.8
rps4-trnT-UGU_p5	TTCCGGGTCTAGGCCTTTTA	TGTTATGTGAGCCCGCTTAG	50.0	50.0	58.0	57.7	548-550 bp	1.0	77.5