Hotspot

trnS-GCU-trnG-UCC

LSC · 8632-9367 · 736 bp

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Pi 0

MarkerSeek score 59.10

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 736
variable_sites: 0
pi: 0
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	ATTAGCAATCCGCCGCTTTA	ACGAATCACACTTTTACCACT	45.0	38.1	58.0	55.5	809-810 bp	1.0	51.6
trnS-GCU-trnG-UCC_p2	ATTAGCAATCCGCCGCTTTA	AGAACGAATCACACTTTTACCAC	45.0	39.1	58.0	57.3	812-813 bp	1.0	50.5
trnS-GCU-trnG-UCC_p3	ATTAGCAATCCGCCGCTTTA	GAACGAATCACACTTTTACCACT	45.0	39.1	58.0	57.3	811-812 bp	1.0	50.5
trnS-GCU-trnG-UCC_p4	ATTAGCAATCCGCCGCTTTA	AACGAATCACACTTTTACCACT	45.0	36.4	58.0	56.2	810-811 bp	1.0	49.7
trnS-GCU-trnG-UCC_p5	ATTAGCAATCCGCCGCTTTA	CAAATTGAAAGACCCCTTAACCA	45.0	39.1	58.0	57.2	849-850 bp	1.0	49.5