Hotspot

rps16-trnQ-UUG

LSC · 6606-7309 · 704 bp

Back to Holboellia

Pi 0.000947

MarkerSeek score 46.90

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 704
variable_sites: 1
pi: 0.000947
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	777-785 bp	1.0	78.4
rps16-trnQ-UUG_p2	TCCACGACTGATCATGTCCT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.1	58.1	799-807 bp	1.0	78.3
rps16-trnQ-UUG_p3	TCATGTCCTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.2	58.1	788-796 bp	1.0	77.9
rps16-trnQ-UUG_p4	AAGTCGCACGTTGCTTTCTA	TAAGGCATCGGGTTTTGGTC	45.0	50.0	58.1	58.2	807-815 bp	1.0	77.6
rps16-trnQ-UUG_p5	TCCACGACTGATCATGTCCT	TAAGGCATCGGGTTTTGGTC	50.0	50.0	58.1	58.2	829-837 bp	1.0	77.5