Hotspot

trnK-UUU-rps16

LSC · 4503-5464 · 962 bp

Back to Holboellia

Pi 0

MarkerSeek score 59.30

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 962
variable_sites: 0
pi: 0
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	TCTACCAACGGTATGGACGA	AAAAAGGCGCTCAACCTACA	50.0	45.0	57.9	58.0	1150-1156 bp	1.0	79.2
trnK-UUU-rps16_p2	AAAGCCGAGTACTCTACCGT	AAAAAGGCGCTCAACCTACA	50.0	45.0	58.2	58.0	1085-1091 bp	1.0	78.9
trnK-UUU-rps16_p3	AAGCCGAGTACTCTACCGTT	AAAAAGGCGCTCAACCTACA	50.0	45.0	58.2	58.0	1084-1090 bp	1.0	78.9
trnK-UUU-rps16_p4	TCTAGCCGCACTTAAAAGCC	AAAAAGGCGCTCAACCTACA	50.0	45.0	58.3	58.0	1099-1105 bp	1.0	78.1
trnK-UUU-rps16_p5	CCGCACTTAAAAGCCGAGTA	AAAAAGGCGCTCAACCTACA	50.0	45.0	58.3	58.0	1094-1100 bp	1.0	78.0