Hotspot

psaC

SSC · 121903-122148 · 246 bp

Back to Hodgsonia

Pi 0.475

MarkerSeek score 75.70

Primer available yes

Label psaC

Feature Metadata

label_name: psaC
feature_type: gene
region: SSC
length_bp: 246
variable_sites: 104
pi: 0.475
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 75.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC_p1	GCTATTGGCCCAGCTATTGT	CCTCCCGCAGTGTACAAAAT	50.0	50.0	58.0	58.2	1241 bp	0.5	58.5
psaC_p2	GCTATTGGCCCAGCTATTGT	AAGGAGCTCGAGTACACAGA	50.0	50.0	58.0	57.8	855 bp	0.5	58.3
psaC_p3	GCTATTGGCCCAGCTATTGT	AGAAGGAGCTCGAGTACACA	50.0	50.0	58.0	57.8	857 bp	0.5	58.3
psaC_p4	GTTATCGCACACATTCCCCT	CCTCCCGCAGTGTACAAAAT	50.0	50.0	58.0	58.2	1649 bp	0.5	58.3
psaC_p5	GCTATTGGCCCAGCTATTGT	AACCCCGTCAGTAAAATGGG	50.0	50.0	58.0	57.8	1004 bp	0.5	58.2