Hotspot

ndhH

SSC · 116203-117384 · 1182 bp

Back to Hodgsonia

Pi 0.454

MarkerSeek score 80.10

Primer available yes

Label ndhH

Feature Metadata

label_name: ndhH
feature_type: gene
region: SSC
length_bp: 1182
variable_sites: 440
pi: 0.454
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.82
markerseek_score: 80.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhH_p1	AAGGAGGGGGAGGAGATTTC	TTGAAGAACTCCCAAAGGCC	55.0	50.0	58.1	58.0	1806 bp	0.5	59.2
ndhH_p2	CGGAAAACGCCAACGATTAC	TTGAAGAACTCCCAAAGGCC	50.0	50.0	58.1	58.0	1611 bp	0.5	58.9
ndhH_p3	AAGGAGGGGGAGGAGATTTC	GGAGCAACACTTGAAATGGC	55.0	50.0	58.1	57.9	2009 bp	0.5	58.6
ndhH_p4	AAGGAGGGGGAGGAGATTTC	AAAGGCCCAGCATATTCAGG	55.0	50.0	58.1	57.9	1793 bp	0.5	58.6
ndhH_p5	GGAGGGGGAGGAGATTTCAT	TTGAAGAACTCCCAAAGGCC	55.0	50.0	58.2	58.0	1804 bp	0.5	58.6