Hotspot

ndhF

SSC · 127011-129260 · 2250 bp

Back to Hodgsonia

Pi 0.436

MarkerSeek score 80.60

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2250
variable_sites: 872
pi: 0.436
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 80.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	ACTGGATCAACCTGTAGGCT	ACGAAGGGGACTAAACAGGA	50.0	50.0	58.0	58.0	2996 bp	0.5	59.5
ndhF_p2	ACTGGATCAACCTGTAGGCT	ACAGGAACAAGAGGGATCCA	50.0	50.0	58.0	58.0	2982 bp	0.5	59.3
ndhF_p3	ACTGGATCAACCTGTAGGCT	AAGAGGGATCCACCGAAGAA	50.0	50.0	58.0	58.0	2974 bp	0.5	59.3
ndhF_p4	ACTGGATCAACCTGTAGGCT	AGGGATCCACCGAAGAAGAT	50.0	50.0	58.0	57.8	2971 bp	0.5	58.2
ndhF_p5	ACTGGATCAACCTGTAGGCT	CTTCTTACCCTCCGGATGGA	50.0	55.0	58.0	58.2	2800 bp	0.5	58.0