Hotspot

ndhA

SSC · 117386-119638 · 2253 bp

Back to Hodgsonia

Pi 0.484

MarkerSeek score 78.40

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2253
variable_sites: 888
pi: 0.484
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.81
markerseek_score: 78.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	ATTGGGGGTTATCAGGACCA	TGCAGCTCGTACTGTTTGTT	50.0	45.0	58.0	58.1	2927 bp	0.5	59.5
ndhA_p2	ATTGGGGGTTATCAGGACCA	TTGCAGCTCGTACTGTTTGT	50.0	45.0	58.0	58.1	2928 bp	0.5	59.5
ndhA_p3	TGGGGGTTATCAGGACCAAT	TGCAGCTCGTACTGTTTGTT	50.0	45.0	58.0	58.1	2925 bp	0.5	59.5
ndhA_p4	TGGGGGTTATCAGGACCAAT	TTGCAGCTCGTACTGTTTGT	50.0	45.0	58.0	58.1	2926 bp	0.5	59.5
ndhA_p5	TAGGGGATCAGGGTGGTTTT	TGCAGCTCGTACTGTTTGTT	50.0	45.0	57.9	58.1	2549 bp	0.5	59.0