Hotspot

rpl32-trnL-UAG

SSC · 119822-121186 · 1365 bp

Back to Hirtella

Pi 0.014
MarkerSeek score 61.50
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
1365
variable_sites
45
pi
0.014
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 ACCGCGTTGAAAGCTTTTTC AGCGTGTCTACCGATTTCAC 45.0 50.0 58.2 58.0 1401-1504 bp 1.0 85.2
rpl32-trnL-UAG_p2 GACCGCGTTGAAAGCTTTTT AGCGTGTCTACCGATTTCAC 45.0 50.0 58.2 58.0 1402-1505 bp 1.0 85.2
rpl32-trnL-UAG_p3 ACCGCGTTGAAAGCTTTTTC CAGCGTGTCTACCGATTTCA 45.0 50.0 58.2 58.0 1402-1505 bp 1.0 85.2
rpl32-trnL-UAG_p4 GACCGCGTTGAAAGCTTTTT CAGCGTGTCTACCGATTTCA 45.0 50.0 58.2 58.0 1403-1506 bp 1.0 85.2
rpl32-trnL-UAG_p5 ACCGCGTTGAAAGCTTTTTC GCATCATTAACCACCGGGAT 45.0 50.0 58.2 58.0 2007-2133 bp 1.0 84.5