Hotspot

trnR-ACG-trnN-GUU

IRb · 113964-114593 · 630 bp

Back to Hirtella

Pi 0.00571

MarkerSeek score 56.30

Primer available yes

Label trnR-ACG-trnN-GUU

Feature Metadata

label_name: trnR-ACG-trnN-GUU
feature_type: igs
region: IRb
length_bp: 630
variable_sites: 7
pi: 0.00571
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-ACG-trnN-GUU_p1	CAGAGGATTAGAGCACGTGG	CCGTTGTTCCTCAGTAGCTC	55.0	55.0	57.8	58.0	765-772 bp	1.0	79.6
trnR-ACG-trnN-GUU_p2	GAACAGATCCAGTGGAGACG	CCGTTGTTCCTCAGTAGCTC	55.0	55.0	57.7	58.0	802-809 bp	1.0	79.0
trnR-ACG-trnN-GUU_p3	CAGAGGATTAGAGCACGTGG	TGGGTTGAGAACGGGAATTG	55.0	50.0	57.8	58.1	806-813 bp	1.0	78.8
trnR-ACG-trnN-GUU_p4	TTTTAGGGTACTCCGGGGAA	CCGTTGTTCCTCAGTAGCTC	50.0	55.0	57.7	58.0	819-826 bp	1.0	78.5
trnR-ACG-trnN-GUU_p5	TTTTTAGGGTACTCCGGGGA	CCGTTGTTCCTCAGTAGCTC	50.0	55.0	57.7	58.0	820-827 bp	1.0	78.5