Hotspot

rpl20-rps12

LSC · 73254-74073 · 820 bp

Back to Hirtella

Pi 0.00341

MarkerSeek score 56.30

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 820
variable_sites: 7
pi: 0.00341
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	TCCGCGCTATATATCCTCGT	GAGGAACATGTACTCGGGTG	50.0	55.0	58.0	57.7	876-892 bp	1.0	78.7
rpl20-rps12_p2	TCCGCGCTATATATCCTCGT	AGGAACATGTACTCGGGTGT	50.0	50.0	58.0	58.4	875-891 bp	1.0	78.2
rpl20-rps12_p3	TCCGCGCTATATATCCTCGT	AAATGTCACCAAATCCCCCG	50.0	50.0	58.0	58.4	920-936 bp	1.0	77.5
rpl20-rps12_p4	AGTAAGTCTTGAATGCGCCC	GAGGAACATGTACTCGGGTG	50.0	55.0	58.3	57.7	938-954 bp	1.0	76.8
rpl20-rps12_p5	AGTAAGTCTTGAATGCGCCC	AGGAACATGTACTCGGGTGT	50.0	50.0	58.3	58.4	937-953 bp	1.0	76.3