Hotspot

psbE-petL

LSC · 68310-69801 · 1492 bp

Back to Hirtella

Pi 0.0102

MarkerSeek score 66.30

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1492
variable_sites: 37
pi: 0.0102
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	AGAACGTTCTCCTGTGCTTC	ACCAATAAATAGGGCTGACGT	50.0	42.9	57.8	57.1	1475-1621 bp	1.0	69.2
psbE-petL_p2	GTTCTCCTGTGCTTCCAGAC	ACCAATAAATAGGGCTGACGT	55.0	42.9	58.2	57.1	1470-1616 bp	1.0	68.9
psbE-petL_p3	AGAACGTTCTCCTGTGCTTC	AGACCAATAAATAGGGCTGACG	50.0	45.5	57.8	57.9	1477-1623 bp	1.0	68.0
psbE-petL_p4	ACCTGCAATGAATAGGGACG	ACCAATAAATAGGGCTGACGT	50.0	42.9	57.7	57.1	1550-1696 bp	1.0	67.7
psbE-petL_p5	CTGCAATGAATAGGGACGGT	ACCAATAAATAGGGCTGACGT	50.0	42.9	57.7	57.1	1548-1694 bp	1.0	67.7