Hotspot

atpB-rbcL

LSC · 57411-58206 · 796 bp

Back to Hirtella

Pi 0.00704

MarkerSeek score 59.70

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 796
variable_sites: 14
pi: 0.00704
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	GCCAATGATTTGAGCGATCC	GCCTTGAATCCAACACTTGC	50.0	50.0	57.6	57.9	922-941 bp	1.0	77.5
atpB-rbcL_p2	GGCCAATGATTTGAGCGATC	GCCTTGAATCCAACACTTGC	50.0	50.0	57.6	57.9	923-942 bp	1.0	77.5
atpB-rbcL_p3	GCCAATGATTTGAGCGATCC	CCAGCCTTGAATCCAACACT	50.0	50.0	57.6	58.1	925-944 bp	1.0	77.4
atpB-rbcL_p4	GGCCAATGATTTGAGCGATC	CCAGCCTTGAATCCAACACT	50.0	50.0	57.6	58.1	926-945 bp	1.0	77.4
atpB-rbcL_p5	TTTTCAAACGCGGAAACTCC	GCCTTGAATCCAACACTTGC	45.0	50.0	57.5	57.9	888-907 bp	1.0	77.0