Hotspot

ndhC-trnV-UAC

LSC · 53172-54322 · 1151 bp

Back to Hirtella

Pi 0.0155

MarkerSeek score 67.30

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 1151
variable_sites: 39
pi: 0.0155
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 67.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	ATGGGGGATAAAACTCCGGA	GCATGTTGGGTCTTTGAAGC	50.0	50.0	58.1	57.9	1328-1367 bp	1.0	83.4
ndhC-trnV-UAC_p2	TCCGGAAATTCGAAATGCCA	GCATGTTGGGTCTTTGAAGC	45.0	50.0	58.2	57.9	1314-1353 bp	1.0	82.9
ndhC-trnV-UAC_p3	ATGGGGGATAAAACTCCGGA	AGAAGGTCTACGGTTCGAGT	50.0	50.0	58.1	57.8	1264-1303 bp	1.0	82.6
ndhC-trnV-UAC_p4	TCCGGAAATTCGAAATGCCA	AGAAGGTCTACGGTTCGAGT	45.0	50.0	58.2	57.8	1250-1289 bp	1.0	82.1
ndhC-trnV-UAC_p5	TGGGGGATAAAACTCCGGAA	GCATGTTGGGTCTTTGAAGC	50.0	50.0	58.3	57.9	1327-1366 bp	1.0	81.6