Hotspot

trnC-GCA-petN

LSC · 28706-29708 · 1003 bp

Back to Hirtella

Pi 0.00618

MarkerSeek score 62.50

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 1003
variable_sites: 14
pi: 0.00618
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	GGGTGTCGCCTGATTAACAA	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.2	58.2	1029-1051 bp	1.0	80.5
trnC-GCA-petN_p2	GGGGGACTGCAAATCCTTTT	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.4	58.2	1065-1087 bp	1.0	79.0
trnC-GCA-petN_p3	CTGCAAATCCTTTTTCCCCG	AGCCCAAGCGAGACTTACTA	50.0	50.0	57.6	58.2	1059-1081 bp	1.0	78.9
trnC-GCA-petN_p4	GGGTGTCGCCTGATTAACAA	AGAGTCCACTTCTTCCCCAT	50.0	50.0	58.2	57.6	1090-1112 bp	1.0	78.7
trnC-GCA-petN_p5	TCCTTTTTCCCCGGTTCAAA	AGCCCAAGCGAGACTTACTA	45.0	50.0	57.5	58.2	1052-1074 bp	1.0	78.2