Hotspot

trnR-UCU-atpA

LSC · 9719-10026 · 308 bp

Back to Hirtella

Pi 0.0286

MarkerSeek score 67.20

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 308
variable_sites: 16
pi: 0.0286
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	TGGAAAAGCGTCCATTGTCT	ATTCGGGAACAGATGGAACG	45.0	50.0	57.7	58.0	432-455 bp	1.0	84.5
trnR-UCU-atpA_p2	TGGAAAAGCGTCCATTGTCT	CGGGAACAGATGGAACGTTT	45.0	50.0	57.7	58.5	429-452 bp	1.0	80.9
trnR-UCU-atpA_p3	TTTGGAATGGAAAAGCGTCC	ATTCGGGAACAGATGGAACG	45.0	50.0	56.9	58.0	439-462 bp	1.0	77.9
trnR-UCU-atpA_p4	TGGAAAAGCGTCCATTGTCT	GCTATTCGGGAACAGATGGA	45.0	50.0	57.7	57.1	435-458 bp	1.0	77.4
trnR-UCU-atpA_p5	TGGAAAAGCGTCCATTGTCT	AGCTATTCGGGAACAGATGG	45.0	50.0	57.7	57.1	436-459 bp	1.0	77.4