Hotspot

trnH-GUG-psbA

LSC · 116-541 · 426 bp

Back to Hirtella

Pi 0.0403

MarkerSeek score 74.60

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 426
variable_sites: 32
pi: 0.0403
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	CTGCCTTAATCCACTTGGCT	ACCTCCAGTTTGATCAGGGA	50.0	50.0	57.9	58.0	848-875 bp	1.0	86.0
trnH-GUG-psbA_p2	CTGCCTTAATCCACTTGGCT	TCAGTGCTATGCATGGTTCC	50.0	50.0	57.9	58.0	874-901 bp	1.0	85.8
trnH-GUG-psbA_p3	ACTGCCTTAATCCACTTGGC	ACCTCCAGTTTGATCAGGGA	50.0	50.0	58.2	58.0	849-876 bp	1.0	85.8
trnH-GUG-psbA_p4	CTGCCTTAATCCACTTGGCT	CAGTGCTATGCATGGTTCCT	50.0	50.0	57.9	58.0	873-900 bp	1.0	85.7
trnH-GUG-psbA_p5	ACTGCCTTAATCCACTTGGC	TCAGTGCTATGCATGGTTCC	50.0	50.0	58.2	58.0	875-902 bp	1.0	85.6