Hotspot

ycf1

SSC · 122060-127825 · 5766 bp

Back to Hippuris

Pi 0.0343

MarkerSeek score 69.20

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5766
variable_sites: 197
pi: 0.0343
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	CTGATTGAAGGCGAAGGGTT	GCAGAATACCGTCACCCATT	50.0	50.0	58.2	58.0	823 bp	1.0	86.3
ycf1_p2	TGAAGGCGAAGGGTTTTTGT	GCAGAATACCGTCACCCATT	45.0	50.0	58.2	58.0	818 bp	1.0	85.9
ycf1_p3	TGATTGAAGGCGAAGGGTTT	GCAGAATACCGTCACCCATT	45.0	50.0	57.7	58.0	822 bp	1.0	85.4
ycf1_p4	CTGATTGAAGGCGAAGGGTT	AAGGCGGATCCGAACAAAAT	50.0	45.0	58.2	58.2	683 bp	1.0	85.3
ycf1_p5	CTGATTGAAGGCGAAGGGTT	TCGAGTTACAGAGACAGGCT	50.0	50.0	58.2	57.8	612 bp	1.0	85.0