Hotspot

trnM-CAU

LSC · 51944-52015 · 72 bp

Back to Hippuris

Pi 0.0694

MarkerSeek score 73.60

Primer available yes

Label trnM-CAU

Feature Metadata

label_name: trnM-CAU
feature_type: tRNA
region: LSC
length_bp: 72
variable_sites: 5
pi: 0.0694
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnM-CAU_p1	GGATGGGTCCCATTTGGTTT	CCGTTGACTCCGGTATCTGA	50.0	55.0	58.0	58.9	136-138 bp	1.0	75.3
trnM-CAU_p2	GGATGGGTCCCATTTGGTTT	CGTTGACTCCGGTATCTGAT	50.0	50.0	58.0	56.8	135-137 bp	1.0	73.3
trnM-CAU_p3	GGATGGGTCCCATTTGGTTT	ACGCAGTTCAAAACGGAAAT	50.0	40.0	58.0	56.9	196-198 bp	1.0	72.5
trnM-CAU_p4	TGGGTCCCATTTGGTTTTCT	CCGTTGACTCCGGTATCTGA	45.0	55.0	57.2	58.9	133-135 bp	1.0	69.5
trnM-CAU_p5	GGATGGGTCCCATTTGGTTT	CCGTTGACTCCGGTATCTG	50.0	57.9	58.0	57.4	136-138 bp	1.0	69.4