Hotspot

ndhG-ndhI

SSC · 116962-117301 · 340 bp

Back to Hippuris

Pi 0.226

MarkerSeek score 76.10

Primer available yes

Label ndhG-ndhI

Feature Metadata

label_name: ndhG-ndhI
feature_type: igs
region: SSC
length_bp: 340
variable_sites: 66
pi: 0.226
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.86
markerseek_score: 76.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhI_p1	AAGACCCGATCCCAGAAAGA	CGAGGCCGAATACACTTTGA	50.0	50.0	58.0	58.0	745-752 bp	1.0	91.6
ndhG-ndhI_p2	AAGACCCGATCCCAGAAAGA	GCTGCCAGATACATTGGTCA	50.0	50.0	58.0	58.0	853-860 bp	1.0	90.7
ndhG-ndhI_p3	AAGACCCGATCCCAGAAAGA	ACGAGCTGCCAGATACATTG	50.0	50.0	58.0	58.1	857-864 bp	1.0	90.5
ndhG-ndhI_p4	AAGACCCGATCCCAGAAAGA	ACAAACAATACGAGCTGCCA	50.0	45.0	58.0	58.1	866-873 bp	1.0	90.0
ndhG-ndhI_p5	AAGACCCGATCCCAGAAAGA	CCTAATTGGCGATTTCCCCA	50.0	50.0	58.0	57.9	461 bp	0.5	59.1