Hotspot

ccsA-ndhD

SSC · 113510-113755 · 246 bp

Back to Hippuris

Pi 0.062

MarkerSeek score 73.30

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 246
variable_sites: 15
pi: 0.062
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 73.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	TGGATATGCTATTTTGGGGTCA	TCAGTTGAAAAGGTTGAAGT	40.9	35.0	57.2	52.8	363-364 bp	1.0	44.2
ccsA-ndhD_p2	TGGATATGCTATTTTGGGGTCA	TCAGTTGAAAAGGTTGAAGTT	40.9	33.3	57.2	53.8	363-364 bp	1.0	44.2
ccsA-ndhD_p3	TGGATATGCTATTTTGGGGTCA	ATCAGTTGAAAAGGTTGAAGT	40.9	33.3	57.2	53.5	364-365 bp	1.0	44.2
ccsA-ndhD_p4	TGGATATGCTATTTTGGGGTCA	TCACTATCAGTTGAAAAGGTTGA	40.9	34.8	57.2	55.8	369-370 bp	1.0	44.1
ccsA-ndhD_p5	TGGATATGCTATTTTGGGGTCA	CACTATCAGTTGAAAAGGTTGAAGT	40.9	36.0	57.2	57.7	368-369 bp	1.0	44.1