Hotspot

trnL-UAG-ccsA

SSC · 112447-112552 · 106 bp

Back to Hippuris

Pi 0.0816

MarkerSeek score 77.40

Primer available yes

Label trnL-UAG-ccsA

Feature Metadata

label_name: trnL-UAG-ccsA
feature_type: igs
region: SSC
length_bp: 106
variable_sites: 8
pi: 0.0816
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 77.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAG-ccsA_p1	TAGACACGCTGCTCTTAGGA	TGCTCTAAGGTTGAAAATATCA	50.0	31.8	57.9	53.2	182-190 bp	1.0	46.9
trnL-UAG-ccsA_p2	GGTAGACACGCTGCTCTTAG	TGCTCTAAGGTTGAAAATATCA	55.0	31.8	57.8	53.2	184-192 bp	1.0	46.8
trnL-UAG-ccsA_p3	GTAGACACGCTGCTCTTAGG	TGCTCTAAGGTTGAAAATATCA	55.0	31.8	57.8	53.2	183-191 bp	1.0	46.8
trnL-UAG-ccsA_p4	AATTGGTAGACACGCTGCTC	TGCTCTAAGGTTGAAAATATCA	50.0	31.8	58.3	53.2	188-196 bp	1.0	46.7
trnL-UAG-ccsA_p5	AAATTGGTAGACACGCTGCT	TGCTCTAAGGTTGAAAATATCA	45.0	31.8	57.8	53.2	189-197 bp	1.0	46.6