Hotspot

ndhF-rpl32

SSC · 110618-111356 · 739 bp

Back to Hippuris

Pi 0.0353
MarkerSeek score 68.10
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
739
variable_sites
26
pi
0.0353
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
68.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 ACGAAAGGTATGATCCACGC TGATGCAGAAGTACGTTTTT 50.0 35.0 58.1 53.5 810-813 bp 1.0 46.1
ndhF-rpl32_p2 ACGAAAGGTATGATCCACGC TTGATGCAGAAGTACGTTTT 50.0 35.0 58.1 53.5 811-814 bp 1.0 46.1
ndhF-rpl32_p3 ACGAAAGGTATGATCCACGC TTTGATGCAGAAGTACGTTT 50.0 35.0 58.1 53.5 812-815 bp 1.0 46.1
ndhF-rpl32_p4 ACGAAAGGTATGATCCACGC TTTTGATGCAGAAGTACGTT 50.0 35.0 58.1 53.5 813-816 bp 1.0 46.0
ndhF-rpl32_p5 ACGAAAGGTATGATCCACGC TTTTTGATGCAGAAGTACGT 50.0 35.0 58.1 53.5 814-817 bp 1.0 46.0