Hotspot

ndhC-trnV-UAC

LSC · 49990-51098 · 1109 bp

Back to Hippuris

Pi 0.0522
MarkerSeek score 75.90
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1109
variable_sites
56
pi
0.0522
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
75.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 CTAGAAAGTTTCTCCGGCCC TGAATCAGAGCACATGGAGC 55.0 50.0 58.0 58.0 1472-1519 bp 1.0 87.0
ndhC-trnV-UAC_p2 CTAGAAAGTTTCTCCGGCCC TTGGTAGTGCTCCTGGATCA 55.0 50.0 58.0 58.0 1505-1552 bp 1.0 86.8
ndhC-trnV-UAC_p3 CTAGAAAGTTTCTCCGGCCC GACAAAAGGTTCGGTCCAGT 55.0 50.0 58.0 58.0 1777-1823 bp 1.0 86.5
ndhC-trnV-UAC_p4 CTAGAAAGTTTCTCCGGCCC CTGACAAAAGGTTCGGTCCA 55.0 50.0 58.0 58.0 1779-1825 bp 1.0 86.5
ndhC-trnV-UAC_p5 CTAGAAAGTTTCTCCGGCCC TGACAAAAGGTTCGGTCCAG 55.0 50.0 58.0 58.0 1778-1824 bp 1.0 86.5